Marleigh+Agner+and+Bailey+Meiner's+ISP

__//** Does Lysol with Bleach Kill more Bacteria than Regular Lysol? **//__ // By Marleigh Agner and Bailey Meiner // // Biology Honors - 1st and 7th Hours //

**ABSTRACT:** This experiment is to determine whether Lysol All Purpose Cleaner with Bleach or Lysol All Purpose Cleaner, without bleach, will kill more bacteria.  **PURPOSE:** **BACKGROUND INFORMATION:** //(Reckitt Benckiser is maker of Lysol)// The reason we are decided to do this experiment is to figure out which Lysol would be more beneficial to use in our houses.  1.) Backed by our expertise in hygiene and killing germs, Lysol has been a trusted brand in creating healthy homes for more than 100 years. We're committed to making a difference, both at home and throughout the community," says Ross Ullman 2.) Reckitt Benckiser (RB) is a world leader in household, health and personal care. Today it is the global No 1 or No 2 in the majority of its fast-growing categories, driven by an exceptional rate of innovation - typically about 35% of net revenue comes from innovations launched in the prior 3 years. 3.) A 2010 Hygiene Home Truths Study sponsored by Lysol(R), results revealed that Americans are neglecting certain areas of the home, particularly in the bathroom and kitchen. By doing so, Americans may be leaving behind dangerous bacteria that increase the risk of illness, as well as cross contamination. And if someone in your home has fallen prey to illness, a lack of proper personal and surface hygiene can keep the germs alive and your household in danger. 4.) Dissolved in boiling water and cooled, agar becomes gelatinous; its chief uses are as a culture medium (particularly for bacteria) and as a laxative, but it serves also as a thickening for soups and sauces, in jellies and ice cream, in cosmetics, for clarifying beverages, and for sizing fabrics.  IV: Type of Lysol we use DV: The amount of bacteria not killed by Lysol We believe that the Lysol with bleach will kill more bacteria than Lysol without bleach. **HYPOTHESIS:** If we use Lysol All Purpose Cleaner with bleach, then it will kill more bacteria than Lysol All Purpose Cleaner without bleach. <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">**MATERIALS:** <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">1.) 1 spray bottle of Lysol All Purpose Cleaner <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">2.) 1 spray bottle of Lysol All Purpose Cleaner with Bleach <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">3.) 8 petri dishes <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">4.) Agar <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">5.) Q-tips <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">6.) Paper towels <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">7.) 2 keyboards <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">**METHODS:** <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">1.) Get a keyboard and swab it for bacteria <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">2.) Put the sample into the petri dish and agar <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">3.) Go back to the surface, and spray one part with Lysol All Purpose Cleaner with Bleach and another part with just Lysol All Purpose Cleaner <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">4.) Wipe the keyboard like you would normally wipe Lysol with paper towels <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">5.) Get new samples of each area and put them on new petri dishes and agar <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">6.) Wait a month for the bacteria to grow <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">7.) Use a grid to see how much bacteria is in each box
 * ANALYSIS:**

We conclude that the Lysol All Purpose Cleaner with Bleach kills a lot more bacteria than Lysol All Purpose Cleaner, without Bleach. The Lysol All Purpose Cleaner, without bleach, still had bacteria while the Lysol with bleach had little to no bacteria in the petri dish. Our first hypothesis was correct. It stated that Lysol All Purpose Cleaner with Bleach would be better than Lysol All Purpose Cleaner, without bleach; our hypothesis is accepted because the Lysol with Bleach did kill more bacteria than the one that didn't.
 * CONCLUSION:**

In the experiment, the bacteria spread over to the control areas in the before samples and the bacteria spread over to the control areas in the after samples of the Lysol without bleach. We could have used more dishes to stop the spread of bacteria into the control areas. We didn't determine how to collect the data at first. We should have determined how to collect data before beginning the experiment. We didn't collect data every day when we could have. We believe that if we would have done a cleaner experiment it would have been easier to take data but the Lysol with bleach would have still killed more bacteria than Lysol without bleach. Our hypothesis is still the same, even considering our limitations: If we <span style="font-family: Arial,Helvetica,sans-serif; line-height: 1.5;">use Lysol All Purpose Cleaner with bleach, then it will kill more bacteria than Lysol All Purpose Cleaner without bleach.
 * LIMITATIONS:**

<span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">**SOURCES:** <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;"> "LYSOL Mission for Health Campaign Launches." PR Newswire 8 Sept. 2010. Gale Student Resources In Context. Web. 1 Dec. 2012. <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">"2010 - A Strong Year for Reckitt Benckiser; Further Growth Targeted In 2011." PR Newswire 11 Feb. 2011. Gale Student Resources In Context. Web. 1 Dec. 2012. <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">"Hygiene; Hygiene Experts Issue Course of Action to Prepare for Cold and Flu." Obesity, Fitness & Wellness Week. 30 Oct. 2010: 739. eLibrary. Web. 20 Dec. 2012. <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">"agar (substance obtained from seaweed)." The Columbia Encyclopedia, 6th ed.. 2012. Retrieved December 21, 2012 from Encyclopedia.com: [] <span style="display: block; font-family: Arial,Helvetica,sans-serif; text-align: left;">**CRITIQUES:** <span style="color: #ee2b2b; font-family: Arial,Helvetica,sans-serif;">Are you going to use the same amount of Lysol on each section?(Angela Kelley) <span style="color: #dd4bc8; font-family: Arial,Helvetica,sans-serif;">I know there are multiple types of Lysol that you can buy, each with a different purpose. Which one will you use and why? (Lena Sander 1-9-13) <span style="color: #2a35bb; font-family: Arial,Helvetica,sans-serif;">How will you actually know which one kills more material because bacteria can be easily seen with only a human eye. (Ayers 1/10/13) <span style="color: #43d649; font-family: Arial,Helvetica,sans-serif;">Do the top three citations have links?? (Kate Mauschbaugh)